MS751-CAS9 cell line

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MS751-CAS9 Order

MS751-CAS9 cell line
Catalog#
YC-C123-Cas9-H
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Datasheet
MS751-CAS9 cell line
Order

Detailed Product Information

Product category
Cas9 Stable Cell Lines
cell name
MS751-CAS9
Cell Morphology
Epithelial-like, adherent
Passage ratio
1:2-1:3
Species
Human
Expression gene
Cas9
Resistance gene
hygro
Construction method
Lentivirus
Mycoplasma test
negative
Culture method
88%MEM+10%FBS+1%NEAA+1%Sodium Pyruvate
Description
Use Cas9 gene expressing lentivirus constructed by Ubigene to infect the MS751 cell line, screen the cells based on the resistance marker and the optimal antibiotic screening concentration explored in advance, resulting in a MS751-CAS9 cell line that can maintain long-term, stable and high expression.
Product Validation Data(RT-QPCR)
Sample Name Target Name Cт Mean ΔCт
MS751-CAS9 Cas9 15.68006420 1.25605297
MS751-CAS9 β-actin 14.42401123
MS751 Cas9 34.88079834 17.69993019
MS751 β-actin 17.18086815
Cutting Efficiency Validation
MS751-CAS9 cell line Product Validation Data(RT-QPCR)
Note: The above figure shows the sequencing peaks of the MS751-CAS9 stable cell pool which is electroporated by KMT2D gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.
Loci STR Info (Sample Cell)
Sample Cell Line:MS751
STR Info (Cell bank)
Cell Line:MS751
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 12121212
D13S317 12121212
D7S820 911911
D16S539 11111111
VWA 16161616
TH01 6666
AMEL XXXX
TPOX 8888
CSF1PO 11121111
D12S391 1522
FGA 2020
D2S1338 2222
D21S11 2930
D18S51 1212
D8S1179 1414
D3S1358 1919
D6S1043 1112
PENTAE 1212
D19S433 1616
PENTAD 99
D1S1656 1217
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Product Advantage

  • Easy to use:Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
    01
  • High-efficient KO:These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
    02
  • High-quality cell:Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.
    03
MS751-CAS9 cell line
MS751-CAS9
Catalog#
YC-C123-Cas9-H
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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